Mining iron: Iron uptake and transport in plants

Mitochondrial iron transport and homeostasis in plants

Iron (Fe) is an essential nutrient for plants and although the mechanisms controlling iron uptake from the soil are relatively well understood, comparatively little is known about subcellular trafficking of iron in plant cells. Mitochondria represent a significant iron sink within cells, as iron is required for the proper functioning of respiratory chain protein complexes. Mitochondria are a si...

Iron transport in Escherichia coli: uptake and modification of ferrichrome.

During the transport of iron as ferrichrome complex into cells of Escherichia coli K-12, the ligand was modified and excreted into the medium. The rate of the formation of the modified product corresponded with the rate of iron transport. The modified product showed a decreased affinity for ferric iron and did not serve as an effective iron ionophore. After all of the ferrichrome had been conve...

Iron transport in Escherichia coli K-12. 2,3-Dihydroxybenzoate-promoted iron uptake.

Copper repletion enhances apical iron uptake and transepithelial iron transport by Caco-2 cells.

The influence of copper status on Caco-2 cell apical iron uptake and transepithelial transport was examined. Cells grown for 7-8 days in media supplemented with 1 microM CuCl(2) had 10-fold higher cellular levels of copper compared with control. Copper supplementation did not affect the integrity of differentiated Caco-2 cell monolayers grown on microporous membranes. Copper-repleted cells disp...

Iron transport in Mycobacterium smegmatis: Uptake of iron from ferric citrate.

In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was not. The uptake system with M. smegmatis was not inhibited by electron transp...